KẾT QUẢ NGHIÊN CỨU TÁI SINH CÂY CHÈ (Camellia sinensis) GIỐNG SHAN CHẤT TIỀN TỪ PHÔI VÔ TÍNH BẰNG NUÔI CẤY MÔ
KẾT QUẢ NGHIÊN CỨU TÁI SINH CÂY CHÈ (Camellia sinensis) GIỐNG SHAN CHẤT TIỀN TỪ PHÔI VÔ TÍNH BẰNG NUÔI CẤY MÔ
Results of research on regeneration plantlets from somatic embryos of tea plant Shan chat tien (Camellia sinensis L.)
Somatic embryo obtained from cotyledon of Shan Chat Tien tea vaiety has potential of germinating and developing mature tea plants. Using MS medium, supplementing with different concentrations of BAP and IBA in different culture stages created new young teas. Somatic embryo was cut into small explants and then cultured on MS medium adding 8mg/1BAP. As a results, 69,34% of explants produced new bud after 20 days. At the multiplying stage, nodal explants with one shoot were cultured in a medium having 3mg/1BAP and 0,3mg/1IBA. Consequently, the height of young tea plants were 4,82cm with 5,7 leaves and obtained 3,53 times higher of multiplying rate after 60 days. Stem explants, which had 3-4 leaves with 2,5-3,0cm length were cultured in MS medium including 3mg/1IBA. As a result, most of roots occurred and grown well after 3 weeks.