XÁC ĐỊNH, THIẾT KẾ MỒI NHẬN BIẾT CANDIDATE GEN KHÁNG RẦY NÂU BPH14 Ở MỘT SỐ GIỐNG LÚA ĐỊA PHƯƠNG CỦA VIỆT NAM VÀ ỨNG DỤNG TRONG LAI TẠO GIỐNG
XÁC ĐỊNH, THIẾT KẾ MỒI NHẬN BIẾT CANDIDATE GEN KHÁNG RẦY NÂU BPH14 Ở MỘT SỐ GIỐNG LÚA ĐỊA PHƯƠNG CỦA VIỆT NAM VÀ ỨNG DỤNG TRONG LAI TẠO GIỐNG
Primer design to determine candidate gene (Bph14) conferring resistance to brown planthopper in Vietnamese local rice varieties and applying for breeding
The brown planthopper (BPH) is one of the major insect pests of many rice growing areas in Vietnam. Based on the sequence data of 36 native rice varieties, a marker namely Bph14add27 was designed to determine candidate gene Bph14 conferring planthopper resistance. The Bph14add27 primer pair was used to amplify the estimate 176bp DNA fragment which was similar with the published resistant rice varieties and 149 bp in others. Based on screening Bph14 resistant candidate gene, two native resistant rice varieties Lua ngoi and OM5629 were used as the donor plants to transfer the resistant gene to commercial rice varieties Bac thom 7 and BC15, respectively. The crossed progenies of backcross generations were tested to determine the presence of Bph14 candidate gene, of which 21 individual plants of BC2F1 population of Lua ngoi Bac thom 7 and 15 plants of BC2F1 population of OM5629 BC15 carrying resistant gene were shown in heterozygote. These lines are ongoing to be used for further investigations.