XÁC ĐỊNH KIỂU GEN PIT1 TRÊN HEO TỪ MẪU LÔNG VÀ ẢNH HƯỞNG LÊN KHẢ NĂNG SINH TRƯỞNG, CHẤT LƯỢNG THỊT HEO

Gene Assisted Selection (GAS) has been applied more popularly in pig selection program in many countries. PIT - 1 or POU1F1 gene is one of main genes of GAS program, because of its effect on growth and carcass quality. 343 hair follicles and fresh semen samples have been collected from many pig herds in Cu Chi, Ho Chi Minh city and O Mon, Can Tho province, in period 2013 - 2014. Four processes of DNA extraction from hair follicle have been examined in order to select the best process for supplying good and stable material for PCR-RFLP. PIT - 1 genotype of these samples has been determined by using two restriction enzymes MspI and RsaI. Average daily gain (ADG), back fat depth (BFD) and loin depth (LD) have been collected by accepted methods for evaluation of relationship between PIT - 1 genotype and growth, carcass quality. Results showed that PIT - 1 genotype from hair follicle sample has been determined with high efficient PCR-RFLP process, C alen from PIT1-MspI genotype have affected positive on growth of pig. It is important basic for applying PIT - 1 genotype in GAS program on pig herd in Southern Vietnam.