ỨNG DỤNG HỆ THỐNG NUÔI CẤY NGẬP CHÌM TẠM THỜI (PLANTIMA® ) TRONG VI NHÂN GIỐNG MÍA Ở VIỆT NAM

In micropropagation, the numbering and quality of the seedlings are highly affected by the shoot multiplication. These experiments we used a temporary immersion system (Playtime®) for shoot multiplication of sugarcane (variety Suphanburi7). The multiplication rate on Suphanburi7 was doubled in comparison with the conventional micro propagation protocol (solid medium). The Basic medium Mutative and Stooge (MS) supplemented with 0.6 mg/l BA, 150 ml/l krypton and 30 g/l sucrose showed the best result for multiplication of the sugarcane shoot. After 20 days culturing we collected the highest number of shoots at the good quality.