TẠO MÔ SẸO VÀ TÁI SINH CÂY IN VITRO TỪ PHÔI NON MỘT SỐ GIỐNG LÚA INDICA

In this study, immature embryos of 18 indica rice cultivars growing in Vietnam such as Khang Dan 18, Huong Xuyen 5, DT36, DT37, DT42, Phieu Huong 1, QR1, Xi-23, IR64, Bac Thom 7, Bac Thom 8, VS1, Lua Thom LT1, Lua Thom LT2, Lua Thom LT10, Tieu Huong 138, Khang Dan dot bien and IR56 were used for callus induction and plant regeneration. It took about 6 weeks to obtain whole regeneration plants that could be transferred to the greenhouse. Callus induction and plant regeneration were carried out on MS (1962) medium containing phytohormones. After 2 weeks of culture, 52.0-72.67% seeds induced embryogenic callus (depending on cultivars). The plant regeneration frequency ranged 10.0-37.14% (depending on cultivars). Frequencies of callus induction and plant regeneration derived from immature embryos depending on immature age and rice cultivars. Survival plant frequency was more than 95% in the greenhouse after 4 weeks. All regenerated plants were fertile and set seeds. There were no morphological variations observed.