TÁCH CHIẾT VÀ PHÂN LẬP PROMOTER OsNAC6 TỪ CÁC GIỐNG LÚA VIỆT NAM

The new trend is continuous activation promoters replaced by induction promoter system that help to effective control the activity of the transform-genes into plant. In this study, we extracted OsNAC6 promoter from the Vietnam rice varieties that grown under artificial drought condition for a week. It is used for inserting into pSK cloning vector in E.coli. Then it will be inserted into the pBIH expression vector and transferred into Agrobacterium tumefaciens strains EHA105 and AGL1. The PCR analysis positive clonies that grown on LB-agar selection medium plates (50mg/l kanamycin, 50mg/l hygromycin) with primers for gus gene (1786bp), hpt gene (514bp) and OsNAC6 promoter (1507bp) tested successfully.