NGHIÊN CỨU XỬ LÝ ĐỘT BIẾN IN VITRO GIỐNG HOA CÚC TÍM NHẬT BẰNG TIA GAMMA

Flowers of Chrysanthemum morifolium ‘Tim Nhat’ variety are purple colour and florets are flat spoon shape. Young flower buds cultured on Murashige and Skoog’s medium supplement with 0.5 mg/l benzyladenine for shoot formation. In vitro internodes with buds were irradiated by لا-radiation (at 5, 10, 15, 20 and 30 Gy). Rooting shoots from لا-irradiated treatments were hardening in the greenhouse and transplanted in the shadehouse for selection. Mutants were obtained at all dose treatments. The mutants have colours from light to dark purple and red-purple, some having flat spoon shaped ray florets similar to the original cultivar, but the different were ray floret structures and size of flowers. Promising mutants were 11 lines (1,5kTN5; 3k59; 3kTN14; 1,5k84; 3k32-1; 1k27-07; 3 kTN12; 0,5k74; 1,5k66; 2k5 and 0,5k89) with new colour and shape different from the original cultivar. Mutation in flower colour was detected from 10 Gy treatment in M1V1 and M1V2 generations. Gamma ray induced flower colour mutant of 1k27-07 line was very attractive colour (light mauve).