NGHIÊN CỨU TÁI SINH GIỐNG ĐẬU TƯƠNG DT2008 VÀ ĐT26 PHỤC VỤ CHO CÔNG TÁC CHỌN TẠO GIỐNG ĐẬU TƯƠNG BẰNG PHƯƠNG PHÁP CHUYỂN GEN

Regeneration from cotyledon and hypocotyl of two soybean cultivars, DT2008 and ĐT26, were studied. Regeneration ratio, shoot induction, shoot elongation and root induction were evaluated on MS media (Murashige Skoog, 1962) supplemented with different concentrations and combinations of phytohormons. MS medium supplemented with 2mg/l BAP and 0,1 mg/l IBA was the most effective on shoot induction of both DT2008 and ĐT26. The regeneration ratio of cotyledon material was 86% with DT2008 cultivar and 88% with ĐT26 cultivar. Regeneration of hypocotyls has ratio of 78,33% and 81,67% for DT2008 and ĐT26, respectively. The induced shoots of the two cultivars were elongated best in MS medium adding 0,5 mg/l GA3 and 0,1 mg/l IAA. Healthy and uniform shoots were rooted in MS medium supplemented with 0,5 mg/l α-NAA.