NGHIÊN CỨU KHẢ NĂNG TÁI SINH IN VITRO TỪ NỐT LÁ MẦM CỦA MỘT SỐ GIỐNG ĐẬU DẢI (Vigna unguiculata L.)

Cowpea is one of the most widely used legumes in the tropical world. In the traditional farming system of Vietnam, cowpea productivity is influenced by many biotic and abiotic factors, especially pod borer, which severely damages cowpea pods in the fields. It is therefore necessary to establish an efficient in vitro regeneration system for successful cowpea improvement programs through genetic engineering. In present study an efficient regeneration protocol was developed for three Vietnamese and three international cowpea cultivars. The experiment was conducted to compare effects of different concentrations of BAP and kinetin on in vitro regeneration from three types of cotyledonary node explants of cowpea. High frequency of shoot regeneration was recorded both in MSB supplemented with BAP or kinetin. However, BAP promoted shoot multiplication better than kinetin. The results displayed that cotyledonary node with both entire cotyledons was the best explant for shoot regeneration, the cotyledonary node with one cotyledon was next-best, and the worst one was cotyledonary node without the cotyledon. BAP at 1 mg/l was optimal for regeneration from cotyledonary node with both cotyledons.