NGHIÊN CỨU ĐÁNH GIÁ TIỀM NĂNG DI TRUYỀN KHÁNG RẦY NÂU Ở MỘT SỐ GIỐNG LÚA ĐỊA PHƯƠNG BẰNG CHỈ THỊ PHÂN TỬ
NGHIÊN CỨU ĐÁNH GIÁ TIỀM NĂNG DI TRUYỀN KHÁNG RẦY NÂU Ở MỘT SỐ GIỐNG LÚA ĐỊA PHƯƠNG BẰNG CHỈ THỊ PHÂN TỬ
Detection of brown planthopper gene resistance of local rice varieties using sequence tagged sites (STS) marker
This study was carried out to evaluate the resistance to brown planthopper of 82 kinds of local rice varieties and identifithe of resistance allen to brown planthopper in rice varieties. The result showed that 5 moderate resistant varieties (score 3), 23 moderate susceptible varieties (score 5), 22 susceptible varieties (score 7) and 32 very susceptible varieties (score 9). The results recorded the amplification of molecular marker RG475FL/RL linked to the resistant gene Bph1..0 was generated to distinguish resistance and susceptibility of 28 rice germplasm accessions obtained from the srceening including TN1 and Ptb33. These products could be digested with HinfI to reveal polymorphism. The final result 4 varieties with resistant homozygote (including Ptb33), 3 varieties with resistant heterozygote and the last 23 varieties without resistance genes (including TN1). Therefor the screening and DNA analysis determined 6 varieties resistant to brown planthopper equivalent Bph1..0. These six rice varieties are the important materials for growing and regenerating of the brown planthopper resistant rice varieties with high yield in Vietnam.