NGHIÊN CỨU ĐA DẠNG KIỂU HÌNH VÀ CÁC MARKER PHÂN TỬ/GEN LIÊN KẾT VỚI TÍNH TRẠNG SỨC SỐNG CÂY CON CHỊU NGẬP Ở GIAI ĐOẠN NẢY MẦM CỦA TẬP ĐOÀN GIỐNG LÚA BẢN ĐỊA VIỆT NAM

In direct-seeded rice ecosystem, seedling vigour under submergence is one of the most important traits for stable stand establishment. In this study, a set of 150 Vietnamese lowland rice cultivars was collected for studying of the “elongation strategy” by using the test tube bioassay screening method. LD (linkage disequilibrium) mapping method was applied to analyze the correlation between marker genotype and phenotype. The results showed that 7 SSR markers were detected association at or 1% either 5% significant level with the seedling vigour under submerged condition by ANOVA analysis. This trait is considered to be controlled by polygenic systems such as a key enzyme in ethylene biosynthesis (OsACSs). The results showed that expression level of OsACS gene family highly induced under submergence by RT-PCR and Real-Time PCR/qPCR analysis. The expression of gene OsACS1 might be a key gene responsible for rice seedling vigour under submergence at post-germination stage. Our results provided useful information for future breeding and genetic study to improve seedling vigour under submergence that supports for directseeded rice ecosystem in Vietnam.