NGHIÊN CỨU CHUYỂN GEN KHÁNG SÂU VÀO PHÔI HỢP TỬ CHƯA TRƯỞNG THÀNH CỦA CÁC DÒNG NGÔ LAI F1

Studies on selection and commercialization of transgenic Bt crops in general and transgenic Bt maize in particular were relatively strong grown in the last few years. However, in Vietnam, the study of transgenic maize met many difficulties due to unidentified source material - the lines / varieties have the ability to regenerate and grow well in nature. In this study, we used immature embryos from three F1 hybrid maize lines (which were the hybrid forms of model lines HR9/HR8 and selected line CM8) as source materials of transformation process. The results showed that the use of those hybrid maize lines were more efficient than model lines HR9/HR8 and selected line CM8 in the transformation of insect resistant gen Cry1A(c) through Agrobacterium tumefaciens. Based on the results of PCR analysis, we had received 27 lines carrying Cry1A(c) gene, 55 lines carrying selectable marker pmi gene, 9 lines carrying both genes in the first generation (T0). Among three hybrid maize lines (HC9, CH9, HC8), HC8 hybrid line had capacity to receive insect resistant gen Cry1A(c) with the highest transformation efficiency (1.41%). In other hand, the T0 transgenic maize lines grew and adapted better than model maize line in nature. The hybrid maize lines carrying Cry1A(c) gen would be suitable materials for developing transgenic maize with insect resistance in Vietnam.