NGHIÊN CỨU ẢNH HƯỞNG CỦA TỪ TRƯỜNG ĐẾN HIỆU QUẢ CHUYỂN GEN THÔNG QUA VI KHUẨN Agrobacterium tumefaciens Ở CÂY NGÔ

The magnetic field is a natural component of our environment, influencing all living organisms. In this study, the effects of magnetic field on bacterial growth and Agrobacterium tumefaciens mediated gene transfer in maize immature embryos was determined. The bacterial growth in log phase was strongest with intensity of magnetic field 0.14T at Southern as well as Northern pole. To estimate the effect of magnetic field, the explants in petri dishes were exposed to 0.085T and 0.14 T magnetic field for cocultivation and regeneration periods at Southern and Northern pole of magnetic field. The results showed that the intensity of magnetic field at 0.14T has positive effect on the cocultivation stage and the transgenic shoot regeneration stage when compared with control explants. The different poles of the magnetic field does not significantly affect to the gene transformation efficiency into maize immature embryo. The considerable increase of gene transformation efficiency in the formula 0.14T/S was 0.21% whereas this ratio was 0.07% in the control fomula.