NGHIÊN CỨU ẢNH HƯỞNG CỦA MỘT SỐ YẾU TỐ MÔI TRƯỜNG ĐẾN KHẢ NĂNG TẠO MÔ SẸO TỪ NUÔI CẤY PHÔI NON TRÊN NGUỒN VẬT LIỆU NGÔ VIỆT NAM

Study to optimize of regeneration system from immature embryos culture plays important role in gene transformation system using Agrobacterium to maize germplasm of Vietnam. The immature embryos (12 days after pollination) of two maize genotypes including hybrid variety LVN154 and inbred line V152N were used to estimate the callus and embryogenic callus induction ability on N6 or MS medium containing different concentration of medium components such as: 2,4-D, AgNO3, sucrose and agar/phytagel. The results showed that callus induction capacity depended on genotype and cultured medium. The hybrid variety LVN154 has the rate of inducted callus and embryogenic callus higher than inbred line V152N. The optimized cultural medium is N6 containing vitamin, 2mg/l 2,4-D, 10 mg/l AgNO3, 30 g/l sucrose, 100 mg/l casein hydrolysate, 25 mM L-prolin, 2.5 g/l phytagel, (pH = 5.8). This is most effective medium to callus induction from immature embryos in both maize hybrid and inbred line.