NGHIÊN CỨU ẢNH HƯỞNG CỦA CHẤT ĐIỀU HÒA SINH TRƯỞNG ĐẾN KHẢ NĂNG TÁI SINH CÂY TỪ PHÔI SOMA PHÁT SINH THÔNG QUA NUÔI CẤY PHÔI NON Ở NGÔ (ZEA MAYS L.)

The plant regeneration of soma embryos decides the success of immature embryos culture and transformation of maize. In vitro the rate of regeneration of maize was low and depended on genotypes, culture conditions and growth regulators that were added on culture medium, specially. The objective of this study was to determine the affection of growth regulators (BAP, kinetin and αNAA) to regeneration ability of maize soma embryos. The results showed that the concentration of BAP and kinetin in the culture medium were increased the rate of shoot induction, in which the medium containing BAP was more efficient than that containing kinetin. The optimized concentration of BAP was 0.5mg/l of media. The suggested components of regeneration medium are: MS + 25mM/l L-prolin + 100 mg/l casein + 10 mg/l AgNO3 + 30g/l sucrose + 2.5g/l phytagen +100ml/l coconut water + 0,5mg/l BAP. The results also documented that α-NAA promoted to root formation and roots elongation of LVN154 and V152N. The suitable concentration of α-NAA was determined is 1mg/l of media. The components of medium using for root induction of plant are MS + 100mg/l myo-inostol + 100mg/l casein+ 30g/l sucrose + 2,5g/l actived charcoal + 2,5g/l phytagen +1 mg/l α-NAA.