MỘT SỐ KẾT QUẢ NUÔI CẤY BAO PHẤN LÚA LAI F1 TẠO DÒNG BẤT DỤC ĐỰC MANG GEN TGMS

This paper presents the results of the research on creation sterile lines rice (Thermo - sensitive genic male sterility) by anther culture of F1 two - lines hybrids rice. The study indicated that a successful haploid technology must depend on a high-efficiently in vitro callus-anther inducing and plant regeneration medium. The highest frequency of callus producing (up to 27,9%) from cultured anther in four hybrids of two-lines varieties CVT 68, LC 212, HYT 102, TH 7-2, was found in Q medium (2mg/l 2,4D + 0,5mg/l Ki + 0,4g CH + 15% (v/v) CW) with 25-30g/l sucrose combine with 15-20g/l maltose. Medium MS with 1mg/l Ki + (1-2)mg/l BAP + 0,5mg/l NAA + 30g/l sucrose + 15% CW was proved the best green regeneration medium with a mean frequency of 15,9%. Green plant was carried out the trial. There are several new TGMS lines carry some good characters that can used for production and research of hybrid rice.