MỘT SỐ KẾT QUẢ NGHIÊN CỨU TẠO DÒNG LÚA NHỊ BỘI KÉP BẰNG XỬ LÝ COLCHICINE

This paper presents the results of the research on induction doubled-haploid rice plant by Colchicine treatment. Rice tillers from four haploids after trimming of roots were treated with Colchicine solution involved different concentrations (0.025%, 0.05%, 0.1% and 0.15%) for different lengths of time (6 h, 12 h, 16 h and 24 h) in an attempt to induce diploidized seeds. A higher Colchicine concentration in combination with longer hours of treatment increased ratio of diploidized seeds, at the same time induced the survival rate treated tillers. Production of diploidized seeds from haploid tillers were proved high effects with treated Colchicine concentrations (from 0.05 to 0.1%) and lengths of time (16 - 24 h) in dependent on varieties.