KỸ THUẬT NHÂN GIỐNG HOA DÃ YÊN THẢO (PETUNIA HYBRYDA) BẰNG PHƯƠNG PHÁP NUÔI CẤY MÔ TẾ BÀO
KỸ THUẬT NHÂN GIỐNG HOA DÃ YÊN THẢO (PETUNIA HYBRYDA) BẰNG PHƯƠNG PHÁP NUÔI CẤY MÔ TẾ BÀO
Micropropagation techniques of Petunia by tissue culture
Petunia is new bedding flower varieties researched and selected by Agriculture Genetics Institute from 2010-2012. Now the propagating for this flower mostly done by cutting and sowing methods, which will produce the undesired effect due to quality of seedlings is not high. The micropropagation techniques of Petunia by tissue culture is very needed to overcome obstacles of traditional propagation. The experiments showed that, the good result for the leaf samples were sterilizated by HgCl2 0,1% within 5 minutes. The optimal medium for shoot regeneration from samples leaf is MS + 0,7 mg/l BAP and MS + 0,4 mg/l BAP + 0,04 mg/l Kinetin +0,5 mg/l Thiamin is good medium for the multiplication of the shoot. The most suitable medium to create roots is MS + 0,4 mg/l α-NAA + 0,5 mg/l Thiamin. In the nursery the best subtrates for planlets is seaweed