CÔNG NGHỆ TẠO DÒNG NGÔ THUẦN BẰNG CÂY KÍCH TẠO ĐƠN BỘI

In vivo haploid induction in maize was learnt at CIMMYT (International Maize and Wheat improvement Center) from december of 2011 to may of 2012. Haploids were generated in maize by using haploid inducer line as a pollinator. Haploid inducers contain R1-nj gene which imparts purple coloration on the embryo and endosperm (facilitates haploid seed identification). All the seedlings were cut root and coleoptile. The seedlings were treated in 0.06% colchicine solution plus 0.05% DMSO (dimethyl sulfoxide) for 12 hours at room temperature. After the seedlings were carefully washed 2 to 3 times with water and transplanted to trays in the greenhouse. When the seedlings grew three to five leaves they were transplanted in the field.