CHỨNG MINH BAC CLONE NHẰM DÒNG HÓA VÙNG CHỨA GEN QUY ĐỊNH MÙI THƠM TRÊN GIỐNG LÚA Oryza sativa L.

Genetic analysis indicated that the aroma was controlled by a single recessive gene named fgr (fragrant rice). In our previous studies based on an BC2F2 population from the cross between OM2517/OM3536 was mapped on chromosome 8. Recently, a BC2F2 population from the same cross was developed and used for the fine mapping of the aroma gene. Molecular marker techniques combined with BAC cloning were used. As a result, two SSR markers (RM223 and SP6), one STS marker (RG28FL-RB) and two BAC (Bacterial Artificial Chromosome) clone: were found to be closely linked to aroma. Finally, the aroma gene was mapped between two markers from BAC cloning at a distance of 1.1 cM from SP6 and 8.5cM from RM223 in population BC2F2 (OM2517/OM3536). Markers for almost all of the aroma have now been analysis in improved varieties and are gradually incorporated into genetic backgrounds of elite cultivars by molecular marker-assisted selection or transformation. It is anticipated that such strategies and efforts would eventually lead to the development of aroma rice.