ỨNG DỤNG PHƯƠNG PHÁP SSR (SIMPLE SEQUENCE REPEATS) TRONG CHỌN TẠO CÁC DÒNG LÚA THƠM

Gene BAD2 with homology to the gene that encodes betaine aldehyde dehydrogenase (BAD) has significant polymorphisms in the coding region of fragrant genotypes relative to non-fragrant genotypes. Here, we show that the evaluation of the aroma of rice by analyzed SSR has great significance in determining the line/aromatic rice varieties for planting serves fragrant rice varieties, good quality later. Through molecular markers will soon aid selection in a rice breeding program. To determine the aroma, we were selected 40 crossed including backcross crossed (BC1F3, BC2F3), single-cross and double-cross from aromatic rice varieties. Analysis based on four markers specific primer ESP, IFAP, INSP and EAP, the results show that: SSR analysis of 198 plants from 40 crossed showed 10 crossed in homozygous for aroma gene and 30 crossed combinations for the genotypes aromatic and non-aromatic. The rate in homozygous for aroma gene in the same crossed was ranged 40% -100%.